Recombinant Kex 2 Protease CAS NO.00-00-0

Kex2 is a Ca²⁺-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃. It is stable in buffer (pH 5.0-6.0). The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition.

packaging：0.5U、10U、50U

Recombinant Kex2 Protease  

 

Catalog Number: Re15

EC: 3.4.21.61

Synonyms: Endoproteinase Lys/Arg-Arg; Kex2 Protease; YSCF Protenase

Source: Recombinant Kex2 Protease is a genetically engineered protein expressed in Pichia pastoris

Storage temperature: 2-8℃

Protein Sequence:The amino acid sequence of recombinant Kex2 Protease is identical to Saccharomyces cerevisiae Kex2

 

1.DESCRIPTION

Kex2 is a Ca²⁺ dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing. But Kex2 can't recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 protease is a genetically engineered protein expressed in Pichia pastoris and with equivalent properties compared to native Saccharomyces cerevisiae Kex2. The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF,TPCK,TLCK inhibition.The optimal pH of Kex2 protease is 9.0. It is stable in buffer (pH 5.0-6.0)

 

2.MAIN FEATURES

Source	Pichia pastoris
Appearance	White or off white powder
Specific activity	≥5.0 units/mg pro.
Purity(SDS-PAGE)	Single major band
Molecular Weight(SDS-PAGE)	24.0±2.4 kDa

UNIT DEFINITION: One unit of Kex2 activity will release 1 μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH 8.0 and 25℃ with Boc-QRR-pNA (Boc-Gln-Arg-Arg-pNA) as the substrate.

 

3.RECOMMEND USAGE

Recommended reaction buffer: pH 7.0-9.0, 50 mM Tris-HCl,2 mM Ca^{2 +} or HEPES, 5 mM Ca^{2 +}. If it is not used immediately after dissolving, it is recommended to dissolve the powder with 20 mM (pH 5.2) NaAc-HAc and 2 mM Ca^{2 +} buffer to make the final concentration is about 1-10 mg/ml,then make suitable packing as your requirement and store at -20℃.Reaction was carried out, using pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca^{2 +} or HEPES, 5 mM Ca^{2 +} buffer.

Note: The optimum reaction pH of the enzyme is pH 9.0 and the optimum stable pH is 5.0-6.0.

 

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage:Recombinant kex2 protease should be stored under 2-8℃ in sealed container.It is stable within 6 months. After dissolved with 20 mM NaAc-HAc,pH 5.2,2mM Ca²⁺, it should be stored under -20℃. It is no activity loss after 5 times repeated freezing and thawing.

Stability of transport:The product is stable by blue ice insulation transport.

 

5.PRODUCT ADVANTAGE

Animal origin free: Recombinant Kex2 protease is no exogenous virus contamination,and any animal origin material is not used in the production process.

Stable quality: Mass production can ensure stable and continuous batch production. It is no difference between the batch and the product quality is stable.

High purity: Higher specific activity. Host protein residues is less than the limits of biological products.

Lyophilized powder: The product is lyophilized powder and is easy to store and transport.

Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory

         requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

Complete quality documents: We can provide relevant regular support files in according to customers’requirement.