Recombinant Carboxypeptidase B CAS NO.9025-24-5

CarboxypeptidaseB

Source	E. Coli
Format	White or white-like or yellowish lyophilized
Additives	Tris salts, NaCl salts and carbohydrates
Protein content	35% ～ 60%
Specific activity	≥170 units/mg pro.
Purity	Single main band on SDS-PAGE
M.W.	35kD
Contaminant activity	Less than 10 ppm of recombinant trypsin.

 

Recombinant Carboxypeptidase B

 

Catalog Number:RCPB01

CAS: 9025-24-5

EC: 3.4.17.2

Storage temperature: 2-8℃

Source: Recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli

Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B

 

1.DESCRIPTION

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into  E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.

 

2.MAIN FEATURES

Source	Recombinant E. coli
Appearance	White or off white, or yellowish powder
Specific activity	≥170 units/mg pro.
Purity(SDS-PAGE)	Single major band
Molecular Weight(SDS-PAGE)	33.8±3.4 kDa

UNIT DEFINITION：One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃,

                                     pH 7.65.

 

3.RECOMMEND USAGE

Prepare 1-10 mg/ml recombinant carboxypeptidase B with sterile water or 25 mM Tris-HCl pH 7.65.

The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0. 

 

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with sterile water or 25 mM Tris-HCl, it should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

 

5.PRODUCT ADVANTAGE

Animal origin free: Recombinant carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.

Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

High purity: Higher specific activity.No other contaminating proteases such as chymotrypsin and carboxypeptidase A.Host protein

                       residues is less than the limits of biological products.

Lyophilized powder: The product is lyophilized powder and is convenient to store and transport.

Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory

       requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

 

6.PRODUCT USAGE

The production of recombinant insulin and its analogues;

the quality test of recombinant antibodies;

the determination of C-terminal amino acids of proteins;

the production of other recombinant polypeptides;

and the enzymatic synthesis of certain specific compounds.

 

7.RELATED PRODUCTS

Recombinant trypsin

Sequencing grade trypsin

Recombinant human trypsin

Recombinant enterokinase